Effects of adding BP5 to the inactivated AIVs on the levels of antigen-specific HI titers (A) and anti-HA IgG antibodies (B). Chicken sera were collected on days 21 and 28 postimmunization, and the serum HI titers and IgG titers were analyzed by HI assay and by ELISA, respectively. The data presented are means ± SD of results from three replicates. *, P < 0.05, and **, P < 0.01 (prime); #, P < 0.05, and ##, P < 0.01 (boost), compared to chickens immunized with inactivated AIVs alone. †, P < 0.05 (prime); §, P < 0.05 (boost), compared to chickens immunized with the commercial H9N2 AIV vaccine.