BP5 significantly stimulates chicken peripheral blood lymphocyte proliferation. Chickens were immunized two times, and chicken peripheral blood lymphocytes were collected on day 28 postimmunization. Proliferative response was evaluated by MTT assay. Data are the means ± SD of results from four separate experiments. *, P < 0.05, compared to results with PBS alone; #, P < 0.05, compared to results with AIVs alone; †, P < 0.05, compared to chickens immunized with the commercial H9N2 AIV vaccine.