Table 1.
Animal groups and the experimental designa
| Group | Vaccination on days 0 and 14b |
|---|---|
| 1 | 500 μl PBS |
| 2 | 400 μl AIVs (107 TCID50/0.1 ml) + 100 μl PBS |
| 3 | 400 μl AIVs (107 TCID50/0.1 ml) + 100 μl BP5 (25 mg/kg/0.1 ml PBS) |
| 4 | 400 μl AIVs (107 TCID50/0.1 ml) + 100 μl BP5 (5 mg/kg/0.1 ml PBS) |
| 5 | 400 μl AIVs (107 TCID50/0.1 ml) + 100 μl BP5 (1 mg/kg/0.1 ml PBS) |
| 6 | 400 μl H9N2 AIV vaccine (107 TCID50/0.1 ml) + 100 μl PBS |
a
All chickens were challenged on day 28.
b
AIVs, inactivated H9N2 avian influenza virus; H9N2 AIV vaccine, commercial H9N2 avian influenza virus vaccine prepared with oil/water as an adjuvant.