Table 2.

Flow cytometric analysis of CD3⁺ T cells and their subsets CD3⁺ CD4⁺ and CD3⁺ CD8⁺ T cells from the peripheral blood lymphocytes of immunized chickens^a

Treatment	% of peripheral blood lymphocytes of type:
	CD3+	CD3+ CD4+	CD3+ CD8+
PBS	35.15 ± 2.14	14.36 ± 1.89	9.22 ± 1.51
AIVs	45.99 ± 1.23	20.87 ± 2.13	13.68 ± 2.12
AIVs + 25 mg/kg BP5	48.78 ± 2.86	20.54 ± 1.38	16.87 ± 1.89
AIVs + 5 mg/kg BP5	58.82 ± 2.48*	26.89 ± 1.56*	22.67 ± 1.78*†
AIVs + 1 mg/kg BP5	61.46 ± 1.61**†	30.44 ± 2.24**	25.01 ± 1.53**†
H9N2 AIV vaccine	56.87 ± 2.31*	28.57 ± 1.79**	16.51 ± 1.35

^aChickens were sacrificed on day 28 after first immunization, and the peripheral blood lymphocytes were collected for immunophenotyping. The data presented are means ± SD of results from four replicates.

^*, P < 0.05, and

^**, P < 0.01, compared with chickens immunized with the inactivated AIVs alone.

^†, P < 0.05, compared with chickens immunized with the commercial H9N2 AIV vaccine.