Table 3.

Detection of AIV copies in the lungs of H9N2 AIV-challenged chickens by a SYBR green 1 real-time PCR^a

Group	No. of lung viral copies (log 10)/ml PBS on day postchallenge:
	1	3	5
PBS	8.6 ± 0.015	7.2 ± 0.036	4.5 ± 0.045
AIVs	7.1 ± 0.023	5.5 ± 0.024	3.1 ± 0.054
AIVs + 25 mg/kg BP5	5.7 ± 0.034*	4.4 ± 0.028*	1.9 ± 0.027*
AIVs + 5 mg/kg BP5	5.4 ± 0.042*	4.0 ± 0.064*	1.5 ± 0.039*
AIVs + 1 mg/kg BP5	5.0 ± 0.041**†	3.1 ± 0.055**†	0.9 ± 0.034**†
H9N2 AIV Vaccine	6.0 ± 0.031*	4.3 ± 0.034*	2.2 ± 0.035*

^aLung samples from individual chickens in each group were collected on days 1, 3, and 5 postchallenge. Each lung sample was diluted to 1 ml with PBS. The titers are presented as numbers of copies per ml PBS. The data presented are means ± SD of results from five replicates.

^*, P < 0.05, and

^**, P < 0.01, compared to chickens immunized with AIVs alone.

^†, P < 0.05, compared with chickens immunized with the commercial H9N2 AIV vaccine.