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. 2011 Aug;77(16):5635–5642. doi: 10.1128/AEM.02875-10

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Fig. 1. — (p)ppGpp analysis of Pseudomonas sp. strain DF41 and its SR derivatives after serine hydroxamate induction. Cells were labeled with ³²P, and nucleotides were extracted and separated by thin-layer chromatography. (A) Lane 1, Pseudomonas aeruginosa strain PAO1; lane 2, Pseudomonas sp. DF41; lane 3, DF41 relA mutant; lane 4, DF41 relA spoT mutant; lane 5, DF41 relA(pUCPrelA) mutant; lane 6, DF41 relA spoT(pUCPrelA) mutant. (B) Lane 1, DF41; lane 2, DF41-469 (gacS mutant); lane 3, DF41-469(pUCP23-gacS); lane 4, DF41-469(pUCPrelA); lane 5, PAO1.