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HPLC analysis of LP produced by Pseudomonas sp. strain DF41, the stringent response mutants, and strains harboring pUCPrelA. LP was extracted from cell-free supernatants of 4-day-old cultures and separated by HPLC. Chromatograms depict the following strains: DF41(pUCP22) (A), DF41-1278(pUCP22) (B), DF41 relA(pUCP22) (C), DF41 relA spoT(pUCP22) (D), DF41 relA(pUCPrelA) (E), and DF41 relA spoT(pUCPrelA) (F). Peaks containing the strain DF41 LP molecule are indicated with arrows.
