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UV chromatograms of methylferulate with XynD-Fae1A digest (A), methylferulate with Axe1-6A digest (B), and methylferulate without enzymes (C). Methylferulate in 50 mM citrate buffer, pH 6 (4 mg/ml), was digested overnight at 37°C, with 0.12% (wt/wt) (enzyme protein/substrate) for Axe1-6A or 1.5% (wt/wt) for XynD-Fae1A.
