Table 1.

Oligonucleotide primers used in this study

Targeta	Primerb	Sequencec
RT qPCR
infB	infBf	5′-TCAGAGCTGGCCACCATGA-3′
	infBr	5′-CAGCATCCAGACGCTGGTT-3′
atpD	atpDf	5′-TGGGTATCTATCCCGCTGTTG-3′
	atpDr	5′-TTGACACGCTGGGCACAAT-3′
rpoB	rpoBf	5′-GAAGACCTTGCTGAGTGGACTGA-3′
	rpoBr	5′-TAGCAGGCTGGTCGAAACG-3′
PRU_0012	12f	5′-TACTGCTATTACTCGCCATCAT-3′
	12r	5′-ATCCATAAATGCACGGCA-3′
PRU_1101	1101f	5′-ACTGATTTTTGCACTGGTGATT-3′
	1101r	5′-AGTTTGCCCGATTGTTTAT-3′
PRU_1396	1396f	5′-ACTAATTGTAGTGCTGGTGAGT-3′
	1396r	5′-TGGCAAAACGTGTAACGTA-3′
PRU_1726	1726f	5′-GACTGGATGTGACTGCCCAT-3′
	1726r	5′-GGCAGCAAACGCTTGGTA-3′
PRU_2033	2033f	5′-TGGTTCGCGCTCAGATTT-3′
	2033r	5′-CCGTTAGCATCCTTCTTCAT-3′
PRU_2212	2212f	5′-CTGATGCTGGCTATGACCAT-3′
	2212r	5′-AACAATGGCTCTGCCTGA-3′
PRU_2584	2584f	5′-AAGCCGAACTTTAGGTAAGA-3′
	2584r	5′-TTCTGTCTGCAACGGTTT-3′
PRU_2630	2630f	5′-TCCTATTATCAGCAGCATTGCT-3′
	2630r	5′-CGGGCAGATAAACAGTTAGTT-3′
PRU_2678	2678f	5′-TGTTTAACTTTGCACCCAAA-3′
	2678r	5′-CCACAAATAATCAGCACGATA-3′
PRU_2707	2707f	5′-CTACCGAACAGGTTGGCAA-3′
	2707r	5′-ATCAGGAGGAAGCGTGGA-3′
PRU_2728	2728f	5′-ACGTCCTGTAGATTATGCAGCA-3′
	2728r	5′-GCAGGATCAGAGATGTTGAA-3′
Heterologous
PRU_2212	2212_HEf	5′-catatgCAGACCGCAAAGAAGTTTACGTTGAACCTGTC-3′
	2212_HEr	5′-ctcgagTTATTTGATGGCAGCCGCAATGATCTCGGCC-3′
PRU_2678	2678_HEf	5′-catatgGAGAATTATCCCTATCGTGCTGATTATTTG-3′
	2678_HEr	5′-ctcgagTTATTTTACTTGTTTTTTGAGCCATAGC-3′
PRU_2707	2707_HEf	5′-gacgacgacaagatgGCCCAGAAACGTAAGGC-3′
	2707_HEr	5′-gaggagaagcccggTTATTTTTTGAACAGATGTGG-3′
PRU_2728 (14)	2728_HEf	5′-catatgAAGAAACTATTAGTAGCGTTATCG-3′
	2728_HEr	5′-ctcgagTTACTTAAAGAGACTCTGAGCCATCTTTTC-3′

^aPrimers were designed and used for the purposes of either RT qPCR or heterologous expression and characterization.

^bf, forward; r, reverse.

^cPrimers were synthesized by Integrated DNA Technologies (Coralville, IA), with restriction enzyme sites indicated by lowercase type.