Table 3.
Locus tag | Product | CE familyb | Cellular locationc | RT qPCRd | Microarraye (Glu; Xyl; AXOS) | REf |
---|---|---|---|---|---|---|
PRU_0012 | Pectin esterase, PecE1 | 8 | Extracellular | 0.93 | 0.61; 0.83; 0.79 | 8.7; 0.07 |
PRU_1101 | Pectin esterase, PecE2 | 8 | Extracellular | 2.18 | 1.37; 1.14; 1.35 | 6.1; 0.12 |
PRU_1396 | Pectin acetyl esterase, PacE1 | Extracellular | 0.94 | 0.26; 0.37; 0.96 | 1.4; 0.01 | |
PRU_1592 | Conserved hypothetical protein | 12 | Cytoplasm | ND | 2.25; 1.77; 0.72 | ND |
PRU_1726 | Carbohydrate esterase | 7 | Periplasm | 11.5 | 2.36; 0.6; 1.26 | 2.7; 0.27 |
PRU_1811 | Polysaccharide deacetylase | 4 | Cytoplasm | ND | 0.61; 0.86; 0.87 | ND |
PRU_2033 | Carbohydrate esterase | Cytoplasm | 7.19 | 6.16; 3.60; 3.14 | 3.8; 0.23 | |
PRU_2212 | Carbohydrate acetyl esterase, AxeA1 | Extracellular | 1.15 | 0.66; 0.58; 1.40 | 13.2; 0.13 | |
PRU_2584 | Carbohydrate esterase | Cytoplasm | 3.17 | 1.02; 1.26; 1.61 | 0.9; 0.02 | |
PRU_2630 | Pectin acetyl esterase, PacE2 | Periplasm | 1.86 | 1.58; 1.65; 1.25 | 5.4; 0.09 | |
PRU_2656 | Isoamylase/esterase | 1 | Periplasm | ND | 1.68; 1.11; 0.90 | ND |
PRU_2678 | Carbohydrate esterase, Axe7A | 7 | Periplasm | 1.71 | 1.81; 1.26; 1.05 | 1.1; 0.02 |
PRU_2694 | Isoamylase/esterase | 1 | Extracellular | ND | 1.62; 1.26; 0.85 | ND |
PRU_2707 | Acetyl-xylan esterase, Axe1-6A | 1/6 | Extracellular | 48.77 | 41.72; 36.01; 4.67 | 1.0; 0.38 |
PRU_2728 | Xylanase/ferulic acid esterase, Xyn10D-Fae1A | 1 | Periplasm | 119.54 | 41.73; 38.10; 4.35 | 1.0; 1.00 |
PRU_2746 | Carbohydrate esterase | 1 | Cytoplasm | 20.36 | 24.97; 18.00; 2.45 | 0.9; 0.15 |
Biochemically characterized proteins are distinguished in boldface type. ND, not determined.
Classified according to carbohydrate esterase (CE) families described in the CAZy database (8) (www.cazy.org).
RT qPCR analysis of the relative expression of each esterase gene during growth on XOSFA,Ac relative to growth on glucose.
Expression of each esterase during growth on XOSFA,Ac relative to that during growth on glucose, xylose, or AXOS.
Expression of each esterase relative to that of xyn10D-fae1A during growth on xylose or XOSFA,Ac.