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. 2011 Aug;77(16):5671–5681. doi: 10.1128/AEM.05321-11

Table 3.

Predicted location and expression of the carbohydrate esterases of P. ruminicola 23a

Locus tag Product CE familyb Cellular locationc RT qPCRd Microarraye (Glu; Xyl; AXOS) REf
PRU_0012 Pectin esterase, PecE1 8 Extracellular 0.93 0.61; 0.83; 0.79 8.7; 0.07
PRU_1101 Pectin esterase, PecE2 8 Extracellular 2.18 1.37; 1.14; 1.35 6.1; 0.12
PRU_1396 Pectin acetyl esterase, PacE1 Extracellular 0.94 0.26; 0.37; 0.96 1.4; 0.01
PRU_1592 Conserved hypothetical protein 12 Cytoplasm ND 2.25; 1.77; 0.72 ND
PRU_1726 Carbohydrate esterase 7 Periplasm 11.5 2.36; 0.6; 1.26 2.7; 0.27
PRU_1811 Polysaccharide deacetylase 4 Cytoplasm ND 0.61; 0.86; 0.87 ND
PRU_2033 Carbohydrate esterase Cytoplasm 7.19 6.16; 3.60; 3.14 3.8; 0.23
PRU_2212 Carbohydrate acetyl esterase, AxeA1 Extracellular 1.15 0.66; 0.58; 1.40 13.2; 0.13
PRU_2584 Carbohydrate esterase Cytoplasm 3.17 1.02; 1.26; 1.61 0.9; 0.02
PRU_2630 Pectin acetyl esterase, PacE2 Periplasm 1.86 1.58; 1.65; 1.25 5.4; 0.09
PRU_2656 Isoamylase/esterase 1 Periplasm ND 1.68; 1.11; 0.90 ND
PRU_2678 Carbohydrate esterase, Axe7A 7 Periplasm 1.71 1.81; 1.26; 1.05 1.1; 0.02
PRU_2694 Isoamylase/esterase 1 Extracellular ND 1.62; 1.26; 0.85 ND
PRU_2707 Acetyl-xylan esterase, Axe1-6A 1/6 Extracellular 48.77 41.72; 36.01; 4.67 1.0; 0.38
PRU_2728 Xylanase/ferulic acid esterase, Xyn10D-Fae1A 1 Periplasm 119.54 41.73; 38.10; 4.35 1.0; 1.00
PRU_2746 Carbohydrate esterase 1 Cytoplasm 20.36 24.97; 18.00; 2.45 0.9; 0.15
a

Biochemically characterized proteins are distinguished in boldface type. ND, not determined.

b

Classified according to carbohydrate esterase (CE) families described in the CAZy database (8) (www.cazy.org).

c

Cellular location of enzyme as predicted by GnegPloc (10, 45).

d

RT qPCR analysis of the relative expression of each esterase gene during growth on XOSFA,Ac relative to growth on glucose.

e

Expression of each esterase during growth on XOSFA,Ac relative to that during growth on glucose, xylose, or AXOS.

f

Expression of each esterase relative to that of xyn10D-fae1A during growth on xylose or XOSFA,Ac.