Fig. 5.

Effect of the conditioned broth on expression of the pcbAB promoter coupled to the reporter lacZ gene. (A) Map of the pDELO construction, containing the promoter of the pcbAB gene fused to the lacZ gene. (B) Quantification of the β-galactoxidase activity in control and conditioned broth-supplemented cultures (AC) of P. chrysogenum Wis 54-1255. The conditioned broth was added (10%) at inoculation time (0 h) to the pDEL0 strain. MilliQ water was added at the same percentage (10%) as for the control. Samples were isolated at 30 h and 60 h of culture, and β-galactosidase activity was measured using protein extracts obtained from those samples. Data represent the means and standard deviations from three independent experiments.