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. 2011 Sep;55(9):4161–4169. doi: 10.1128/AAC.00111-11

Fig. 1.

Fig. 1.

Construction of ZmA biosynthesis-related gene cluster BAC clone linkage group and comparison of ZmA gene clusters from three strains. The ZmA biosynthesis genes from B. cereus UW85 and AH1134 that were discovered by Thomas' group are shown at the top (quoted as described in reference 18). The region of zma16Bc is indicated by an ellipse frame on the ZmA gene cluster of strain YBT-1520. The fragments cloned from strain YBT-1520 in nine BAC clones are indicated by black lines under the gene cluster. The names of the clones and the sizes of the DNA fragments are described above the line. Red arrows indicate genes potentially involved in ZmA biosynthesis, blue arrows indicate genes potentially involved in ZmA self-resistance, the green arrow indicates a gene potentially involved in regulation, yellow arrows indicate transposase genes (tnp), gray arrows indicate ORFs without clear functions, and purple arrows denote genes involved in kanosamine biosynthesis in UW85 (kabRABCD). The target position of the two primer pairs F1/F2 and F3/F4 are indicated by two black arrows.