Table 1.
Bacterial strains and plasmids used in this study
| Species and strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| E. coli DH10B | Δ(mrr hsdRMS mcrBC) mcrA recA1 | New England Biolabs |
| E. herbicola LS005 | Indicator strain for ZmA production | 31 |
| B. cereus UW85 | Produces ZmA | 31 |
| B. thuringiensis | ||
| YBT-1520 | B. thuringiensis subsp. kurstaki | 38 |
| BMB171 | Acrystalliferous mutant of B. thuringiensis | 13 |
| BMB1212 | BMB171 with plasmid pEMB1212, carrying zmaR | This study |
| BMB1236 | Mutant of strain YBT-1520 in which the orf123 gene was knocked out | This study |
| BMB1237 | BMB1236 with plasmid pEMB1237, in which the knocked-out orf123 gene was complemented | This study |
| Plasmids | ||
| pBeloBAC11 | BAC cloning vector for E. coli | 21 |
| pHT304 | E. coli and B. thuringiensis shuttle vector | 1 |
| pHT304-ts | pHT304 derivative with a temp-sensitive Bacillus replicon; Ermr for B. thuringiensis | 20 |
| pDG780 | Vector with Kanr for B. thuringiensis | BGSC |
| pEMB0557 | E. coli-to-B. thuringiensis shuttle BAC vector; ori60, Ermr for B. thuringiensis | 19 |
| pEMB0603 | E. coli-to-B. thuringiensis shuttle BAC vector; ori44, Kanr for B. thuringiensis | This study |
| pEMB1212 | 1.2-kb PCR fragment containing zmaR cloned into pHT304 at SphI/BamHI sites | This study |
| pEMB1231 | 2.9-kb PCR fragment containing zmaWXY cloned into pHT304 at SphI/BamHI sites | This study |
| pEMB1232 | 33.7-kb 4B1 NotI fragment containing zmaR cloned into pEMB0603 at NotI site | This study |
| pEMB1236 | Interrupted orf123 gene cloned into pHT304-ts | This study |
| pEMB1237 | orf123 gene cloned into pHT304 | This study |
a
Kanr, kanamycin resistance; Ermr, erythromycin resistance.