Fig. 1.

Yeast membrane-associated two-hybrid screening for inhibitors of Gag-Gag interaction. (A) Schematic representation of Gag expression plasmids used for yeast SOS recruitment system. The full-length gag gene of HIV-1 (HXB2 strain) was expressed by yeast expression plasmids pMyr and pSOS: pMyr contains the yeast inducible promoter for the GAL1 gene and the URA3 gene as a selective marker and pSOS contains the constitutive promoter for the yeast ADH gene and the LEU2 gene as a selective marker. (B) Principle of yeast membrane-associated two-hybrid assay based on SOS recruitment system. The schematic illustration was adapted with permission from the manuals for the CytoTrap yeast system (Agilent Technologies, Inc.; http://www.genomics.agilent.com/CollectionSubpage.aspx?PageType=Product&SubPageType=ProductDetail&PageID=1311).