Fig. 3.

Structural analysis of HCMV DNA polymerase. (A) The proposed backbone displacement of the homology 3D structure of the HCMV DNA polymerase is presented along with the DNA template. The four colors represent the four TLS groups moving independently from one another during TLS simulation (blue, residues 27 to 163; green, residues 164 to 312; red, residues 313 to 568; magenta, residues 569 to 1227). The width of the strand indicates the predicted amplitude of movement. The proposed functional assignments to these groups are indicated. (B) Model of the HCMV polymerase in editing mode with GCV, primer, and template DNA. The predicted catalytic site is also indicated. The positions of the mutations selected with GCV from the clinical isolate are indicated in blue, whereas those selected from AD169 are in yellow. The backbone color pattern is the same as for panel A. (C) Replicative model of HCMV polymerase with template and primer DNA and dTTPs. Locations of the mutations selected with FOS are indicated in either red or blue to discriminate between the two patterns of emergence that were observed. The backbone color pattern is the same as for panel A.