Fig. 4.

Q24DA inhibits V. cholerae virulence gene expression. (A) Strain C7258 was grown in AKI medium (13) (control) and in the same medium containing Q24DA (10 μg/ml). CT and the TCP major subunit TcpA were detected by GM₁-ELISA and Western blotting, respectively. BI, band intensity. (B) Three rabbit ileal loops were inoculated with pure CT (20 μg), strain C7258 (WT), C7258 containing 10 μg/ml Q24DA, or C7258 Mot⁻. Results are expressed as amounts of fluid accumulation (FA) in ml per cm of loop. *, P = 0.026; **, P = 0.029.