Table 1.
Strains, plasmids, and primers used for this study
| Strain, plasmid, or primer | Relevant description or sequence (5′–3′) | Reference(s) or source |
|---|---|---|
| Strains (pathogenic Vibrio strains) | ||
| V. cholerae C7258 | Wild type, O1 El Tor biotype, Ogawa | Peru (1991), clinical isolate |
| V. cholerae C7258 Mot− | C7258 ΔmotY | This study |
| V. cholerae C7258 Fla− | C7258 ΔflaA | This study |
| V. cholerae O395 | O1, classical biotype, Ogawa | 16 |
| V. cholerae O395ΔAB | O395 ΔmotA ΔmotB | 16 |
| V. parahaemolyticus B22 | Wild type; Fla+ Laf+ | 3 |
| V. parahaemolyticus LM5674 | Wild type; Fla+ Laf+ | 27, 29 |
| V. parahaemolyticus LM1017 | Fla+ Laf− | 27, 29 |
| V. parahaemolyticus LM5392 | Fla− Laf+ | 27, 29 |
| V. parahaemolyticus LM7890 | Fla+Laf− | 27, 29 |
| V. vulnificus LAM624 | Wild type | 59 |
| O395ΔAB Phes | O395ΔAB containing pLM2058 | This study |
| O395ΔAB Pher | O395ΔAB containing pLM2059 | This study |
| Plasmids | ||
| pLM2058 | Cosmid containing wild-type V. parahaemolyticus motor genes | 27 |
| pLM2059 | Cosmid containing phenamil resistance motor genes | 27 |
| pRK2013 | Helper plasmid for mobilization of non-self-transmissible plasmids (Kmr) | |
| Oligonucleotide primers | ||
| FlaAF | GATCGCATATGACCATTAACGTAAATACCAA | |
| FlaA-R3 | TACTAGTGCATCTCCCGTGATGCACTGCAATAACGAGAT | |
| ToxT189 | GCCCTCTATTCAGCGATTTTCTTTG | |
| ToxT400 | GCCCCTCCATAGCATCAAGATCATC | |
| MotA212 | ATGCTCCCGAAGATCTGATTGCCA | |
| MotA56 | ACCAGTGTCCCGATCATCCCCAT | |
| MotB433 | CAACAGCAGCAAGCACAAGCGATG | |
| MotB651 | CGCACTTTACCGGGAATGTCTTTG | |
| MotX240 | TATGCTGGCTTGGGGAGTGTGTGT | |
| MotX476 | CATCTTCATAATCGAGCGGACTGC | |
| MotY362 | CCGCGTGGTCGTTACTCAGTGAA | |
| MotY572 | GACGCTTTATTCAACTCAACACTGG | |
| RecA578 | GTGCTGTGGATGTCATCGTTGTTG | |
| RecA863 | CCACCACTTCTTCGCCTTCTTTGA |