Table 2.

Spiking of fecal samples with NDM-1-producing enterobacterial isolate

Isolate no.	Bacterium	IMP MIC (μg/ml)	Lowest limit of detection in water (CFU/ml)		Lowest limit of detection in stoola (CFU/ml of stool)a		Lowest limit od detection of NDM-1 qPCR
			ChromID ESBL	CHROMagar KPC	ChromID ESBL	CHROMagar KPC	CFU/ml of stool	No. of DNA copies/ml of stoolb
23	K. pneumoniae A	>32	1 × 101	1 × 101	2 × 101	1 × 102	101	1 × 102
24	K. pneumoniae B	>32	2 × 101	1 × 101	2 × 101	5 × 101	2 × 101	2 × 102
25	K. pneumoniae D	>32	3 × 101	2 × 101	2 × 101	2 × 101	101	4 × 102
26	K. pneumoniae F	>32	1 × 101	2 × 101	1 × 101	2 × 101	2 × 101	2 × 102
27	K. pneumoniae G	2	2 × 101	3 × 102	1 × 102	4 × 103	101	2 × 102
28	K. pneumoniae I	>32	1 × 101	3 × 101	2 × 101	6 × 101	101	6 × 102
29	C. freundii A	>32	1 × 101	1 × 101	1 × 101	1 × 101	3 × 101	6 × 102
30	E. coli A	6	2 × 101	4 × 101	2 × 101	3 × 102	101	2 × 102
31	E. coli B	3	1 × 101	2 × 103	1 × 101	3 × 103	101	2 × 102
32	E. cloacae A	8	4 × 101	3 × 102	2 × 101	8 × 101	3 × 101	103

^aCFU/ml of stool. One milliliter of stool contains 100 mg of stool. Values were calculated using the standard curve (Fig. 2B).

^bCopies of bla_NDM-1 gene/ml of stool. One milliliter of stool contains approximately 100 mg of stool. Values were calculated using the standard curve (Fig. 1B).