Table 5.
Comparative analysis of CAP susceptibility, cell membrane fluidity, and cell wall thickness of the DAPs parental strains and the 7 DAPr strains with mutations in mprF (with or without yycG mutations)a
| Parameter | DAPs strains (n = 10) | DAPr strains (n = 7) | P value |
|---|---|---|---|
| % survival after 2 h of exposure to CAP (concnb) | |||
| tPMP (0.50) | 31 ± 32 | 71 ± 20 | 0.0112 |
| tPMP (0.25) | 43 ± 29 | 86 ± 10 | 0.016 |
| hNP-1 (10) | 20 ± 21 | 51 ± 21 | 0.0085 |
| hNP-1 (5) | 30 ± 22 | 62 ± 14 | 0.0041 |
| PMB (40) | 12 ± 17 | 37 ± 23 | 0.0207 |
| PMB (20) | 44 ± 23 | 64 ± 15 | 0.0599 |
| Cell membrane fluidity (PI value) | 0.369 ± 0.05 | 0.321 ± 0.04 | 0.0441 |
| Cell wall thickness (nm) | 33.84 ± 4.1 | 38.60 ± 3.3 | 0.0218 |
a
Values are means ± SDs.
b
Concentrations are in micrograms per milliliter.