Figure 4.

 Rescue of the ptp-2(op194) phenotypes by germ-line transformation. YAC filter hybridization and sequence analysis by the C. elegans genome sequencing project placed ptp-2 ∼10 kb to the right of lin-4 (top). Cosmids containing wild-type genomic ptp-2 and the plasmid pRF4, which contains the marker mutation rol-6(su1006), were microinjected into ptp-2(op194) unc-4(e120)/dpy-10(e128) unc-104(e1265) II adult hermaphrodites in an attempt to establish fertile obligate-transgenic lines (see Materials and Methods). Numbers to the right indicate the number of rescued lines/number of stable transgenic lines established. Of the cosmids microinjected, the relative boundaries of the F59G1 3′ end and F28B12 5′ end were confirmed by PCR; however, the exact boundaries of C52G9, the 5′ end of F59G1, and 3′ end of F28B12 are approximated.