Table 1.
Antiviral pressure (fold EC50) | Fold increaseb compared to WT | EC50 ± SDb (μM) | NS3 mutation(s) (aa 22-336)c | Critical phased |
---|---|---|---|---|
1 | 2 | 0.033 ± 0.008 | S174S/C | N |
2 | 16 | 0.24 ± 0.08 | D168D/E/V, S174S/C | N |
5 | 13 | 0.19 ± 0.09 | D168D/E/V, S174S/C | N |
25 | 67 | 1 ± 0.4 | D168V, E176E/G | Y |
125 | 200 | 3 ± 0.5 | D168V, S174S/C, E176E/G | Y |
2-5 | 17 | 0.26 ± 0.007 | D168D/E/V, S174S/C | N |
2-25 | 61 | 0.92 ± 0.3 | D168V, S174S/C, E176E/G | N |
2-125 | 133 | 2 ± 0.2 | D168V, S174S/C, E176E/G | N |
5-25 | 56 | 0.84 ± 0.005 | D168D/V, S174S/C, E176E/G | N |
5-125 | 133 | 2 ± 0.6 | D168V, S174S/C, E176E/G | N |
25-125 | 133 | 2 ± 0.3 | D168V | N |
2-5-25 | 133 | 2 ± 0.6 | D168V, S174S/C | N |
2-5-125 | 133 | 2 ± 0.6 | D168V, S174S/C | N |
2-25-125 | 133 | 2 ± 0.5 | D168V, S174S/C, S280S/A | N |
5-25-125 | 200 | 3 ± 0.04 | D168V, S174S/C, E176E/G, S280S/A | N |
2-5-25-125 | 267 | 4 ± 0.7 | D168V, S174S/C | N |
EC50, 0.015 ± 0.01 μM; CC50, 22.6 ± 0.1 μM.
Data are mean values for 2 independent determinations of the antiviral phenotype.
Major resistance mutations are indicated in boldface. aa, amino acids.
Y, yes; N, no.