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. 2011 Sep;55(9):4311–4319. doi: 10.1128/AAC.00644-11

Fig. 4.

Fig. 4.

(a) Huh7 cells harboring the 9B HCV subgenomic replicon were stably transfected with USP18 shRNA as shown. After 24 h of treatment with a serial dilution of IFN-α2a, the number of replicon RNA genomes was measured by using RT-PCR and corrected to actin. The numbers in square brackets represent the EC50s calculated from the IFN dose-response curves generated with GraphPad Prism software (version 5.01). Error bars represent ranges of replicate data points. (b) The same cells as those described above were treated with 1.8 IU/ml IFN-α2a for 4, 8, and 24 h, and RNA was extracted for RT-PCR analysis for actin and 2-5 OAS. The extent of induced 2-5 OAS expression corrected for actin for each shUSP18 stable cell line is plotted against time. The numbers in square brackets represent USP18 expression levels.