Figure 3.

 Replication kinetics after a pulse of Cdc7 activity. (A) Cells released at 23°C from a cdc block and returned to 37°C after 3.5 min at 23°C. (B) Control cells released from the cdc7 block and kept at 23°C. Replication kinetics of two early-replicating standard fragments, containing ARS305 (solid line with open rectangles) and ARS1 (broken line with filled ovals), and two late-replicating standard fragments, R11 (broken line with filled rectangles) and a fragment containing ARS1412 (solid line with open triangles) are shown.