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Two-dimensional gel analysis of early and late replication origin activation at semipermissive temperatures for the cdc7-1 allele. (Top) Blots probed for replication intermediates of a fragment containing early origin ARS306. (Bottom) Blots probed for the late origin ARS1412. Left panels show DNA from an asynchromous culture grown at permissive temperature (23°C). Center and right panels show blots made with DNA from cultures grown at semipermissive temperatures (26.5°C and 27°C).
