Table 2.

Percent replication of early and late plasmids and chromosomal sequences after a pulse of Cdc7 activity

Length of release at 23°C	ARS305		R11		p306.10 (early)		p12 (late)
	65 min	80 min	65 min	80 min	65 min	80 min	65 min	80 min
3.5  min	57	59	22	26	—	—	—	—
5 min	56	60	26	32	63	60	—	—
5 min	63	66	28	33	—	—	13	13
6 min	64	66	33	41	65	63	—	—
6 min	63	67	29	38	—	—	13	17
7.5  min	72	76	48	53	71	71	—	—
7.5  min	77	75	54	58	—	—	18	21

Samples were taken 65 and 80 min after brief release from a cdc7 block. The 3.5-min release length data are from the experiment shown in Fig. 3, in which the culture contained no plasmid. For 5-, 6-, and 7.5-min release lengths, the top values correspond to a culture containing p306.10, and the bottom values to a culture containing p12. The 7.5-min release data are taken from Fig. 5.