Table 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant propertiesa | Source or reference |
|---|---|---|
| Strains | ||
| R. solanacearum | ||
| GMI1000 | Wild-type strain | 33 |
| RS6 | RSc1806::pHiK002 in GMI1000 | This study |
| E. coli | ||
| TOP10 | F−mcrA Δ(mrr–hsdRMS–mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| BW25113 | K-12 derivative: ΔaraBAD ΔrhaBAD | 6 |
| BW25113-2 | BW21533 harboring pIJ790 and pHiK001 | This study |
| Plasmids | ||
| pJET1.2 | Cloning vector; Ampr | Fermentas |
| pIJ790 | Lambda-Red (gam, beta, exo), araC, rep101(Ts); Cmr | 22 |
| pAphA-3 | Kanamycin resistance cassette; Kmr | 27 |
| pHiK001 | pJET1.2 carrying 1.7-kb RSc1806 fragment | This study |
| pHiK002 | pHiK001 carrying RSc1806::Kmr | This study |
a
Abbreviations: Ampr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance.