Table 1.
Primers used for overexpression and quantitative PCR of the feruloyl esterases
| Primera | Sequenceb |
|---|---|
| faeI-F | CCGCATATGGCAATTACGCCAGTAAT |
| faeI-R | CGGGATCCTTATTTAAGCACTACATTAGC |
| faeII-F | CGGGATCCATGAAAGAAATATCAGATGC |
| faeII-R | CCGCTCGAGTTATTTTGTATATTTATCAGC |
| faeIII-F | CCGCATATGGTAGGGGTAGAAAAG |
| faeIII-R | CGGGATCCTTGGCAAAGATTCTAACTT |
| Q16s-F | TACACACCGCCCGTCACAC |
| Q16s-R | CAGCCGCACCTTCCGATAC |
| QfaeI-F | ATACCACCAGCAGGATAT |
| QfaeI-R | TTCATTACCACCAATACCAT |
| QfaeII-F | ACACCAAGTAGAGATAGCAATA |
| QfaeII-R | GCCTACAACCTCATCACT |
| QfaeIII-F | AGTAGTTGGCAGAGGATT |
| QfaeIII-R | ATGGCATTGACCTTCTTC |
a
The Q prefix identifies the primers designed for quantitative PCR.
b
Restriction sites are underlined.