Table 2.
Substrate specificities of FaeI, FaeII, and FaeIII
| Substratea | Sp act (U mg−1)b of: |
||
|---|---|---|---|
| FaeI | FaeII | FaeIII | |
| Maize cob | 0.05 ± 0.01 | 0.10 ± 0.00 | 0.06 ± 0.00 |
| Maize cobc | 2.03 ± 0.12 | 2.64 ± 0.18 | 0.63 ± 0.07 |
| MFA | 3.78 ± 0.10 | 38.76 ± 1.3 | 56.88 ± 0.49 |
| MCA | 1.47 ± 0.04 | 18.44 ± 0.54 | 32.58 ± 1.12 |
| MpCA | 1.1 ± 0.01 | 32.65 ± 0.67 | 34.19 ± 0.98 |
| MSA | 3.66 ± 0.01 | 29.04 ± 0.29 | 37.51 ± 0.67 |
| CGA | ND | 5.74 ± 0.07 | 21.73 ± 0.28 |
| pNP esters by chain length | |||
| C2 | 0.16 ± 0.01 | 0.36 ± 0.01 | 0.25 ± 0.01 |
| C4 | 0.29 ± 0.01 | 0.44 ± 0.01 | 0.16 ± 0.01 |
| C6 | 0.15 ± 0.03 | 0.31 ± 0.02 | 0.15 ± 0.01 |
| C8 | 0.07 ± 0.01 | 0.23 ± 0.02 | 0.06 ± 0.01 |
| C10 | 0.03 ± 0.01 | 0.02 ± 0.01 | 0.01 ± 0.01 |
a
MFA, methyl ferulate; MCA, methyl cinnamate; MpCA, methyl p-coumarate; MSA, methyl sinapate; CGA, chlorogenic acid; C2, p-nitrophenyl (pNP)-acetate; C4, pNP-butyrate; C6, pNP-caproate; C8, pNP-octanoate; C10, pNP-caprate.
b
Values are given as means ± standard deviations. ND, not determined.
c
Activity calculated as ferulic acid (μmol) released per mg of protein in 6 h.