Table 3.
Kinetic parameters of FaeI, FaeII, and FaeIII on model substratesa
| Substrate | FaeI |
FaeII |
FaeIII |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Km (mM) | kcat (s−1) | kcat/Km (s−1 mM−1) | Km (mM) | kcat (s−1) | kcat/Km (s−1 mM−1) | Km (mM) | kcat (s−1) | kcat/Km (s−1 mM−1) | |
| MFA | 4.78 ± 0.46 | 8.46 ± 0.55 | 1.77 | 0.36 ± 0.03 | 645.94 ± 14.75 | 1,794.28 | 0.03 ± 0.00 | 2,130.89 ± 33.28 | 71,029.64 |
| MCA | 2.50 ± 0.28 | 2.34 ± 0.22 | 0.94 | 0.16 ± 0.01 | 232.72 ± 6.41 | 1,454.49 | 1.06 ± 0.03 | 330.90 ± 4.06 | 312.17 |
| MpCA | 13.76 ± 0.82 | 7.78 ± 0.40 | 0.57 | 0.51 ± 0.03 | 310.51 ± 17.68 | 608.85 | 1.26 ± 0.08 | 538.62 ± 59.99 | 427.48 |
| MSA | 6.58 ± 0.38 | 12.70 ± 0.97 | 1.93 | 0.18 ± 0.01 | 753.63 ± 11.88 | 4,186.81 | 0.63 ± 0.03 | 566.97 ± 7.74 | 899.96 |
a
Values are given as means ± standard deviations or ratios; substrate abbreviations are defined in Table 2.