Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Escherichia coli JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′ [traD36 proAB+lacIqlacZΔM15] | Promega |
| Burkholderia contaminans | ||
| MS14 | Wild-type strain | 13 |
| MS14MT15 | ambR2::Tn5 derivative of MS14; Kmr | 13 |
| MS14GG44 | ambR1::nptII derivative of MS14; Kmr | 14 |
| MS14MT24 | ocfH::Tn5 derivative of MS14; Kmr | This study |
| MS14GG78 | ocfJ::nptII derivative of MS14; Kmr | This study |
| Plasmid/fosmid | ||
| pSL604 | EZ-Tn5 carrying the ∼1-kb genomic DNA of MS14MT24; Kmr | This study |
| pBluescript II SK | Cloning vector; Apr | Stratagene |
| pBR325 | Cloning vector; Cmr Tcr Apr | 31 |
| pMLS7 | Expression vector of Burkholderia; Tpr | 21 |
| pGEM-T Easy | Cloning vector; Apr | Promega |
| pBSL15 | Kanamycin resistance gene cassette; Kmr | 1 |
| pGG20 | pGEM-T Easy carrying 4-kb PCR product containing the ocfJ gene; Apr | This study |
| pGG21 | pGG20 with the nptII insertion in ocfJ at the BamHI site; Kmr Apr | This study |
| pGG22 | pBR325 carrying the 5-kb EcoRI fragment containing ocfJ gene disrupted by nptII; Cmr Tcr Kmr | This study |
| pGG23 | pGEM-T Easy carrying 4.5-kb PCR product containing the intact ocfJ gene; Apr | This study |
| pGG24 | pMLS7 carrying 4.5-kb HindIII fragment harboring the intact ocfJ gene; Tpr | This study |
| 4G5 | pCC1FOS carrying 40-kb genomic DNA of MS14; Cmr | This study |
a
Kmr, kanamycin resistance; Apr, ampicillin resistance; Tpr, trimethoprim resistance; Cmr, chloramphenicol resistance; Tcr, tetracycline resistance.