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. 2000 Apr 15;14(8):951–962.

Figure 5.

Figure 5

Bdf1 coimmunoprecipitates with TFIID components. (A) Bdf1 coimmunoprecipitates with Taf67. Extract (Taf67HA) from strain YSB458 carrying an epitope-tagged Taf67 was immunoprecipitated (IP) with preimmune serum (PIS), mAb 12CA5 (α-HA), or α-Bdf1 polyclonal antibody as indicated. The signal in total extract (Sup) is also shown. Wild-type (WT) extract serves as a negative control for the epitope tag. (B) Bdf1 coimmunoprecipitates with TFIID components. Extracts from a wild-type (WT, YSB382) or bdf1Δ strain (YSB496) were immunoprecipitated with preimmune serum (PIS), α-TBP antibody, or α-Bdf1 antibody as indicated. bdf1Δ plus GST–Bdf1(C) is bdf1Δ extract with 125 ng of recombinant GST–BDF1(C) protein added before immunoprecipitation. The precipitates were resolved on SDS-PAGE, blotted, and probed with indicated antibodies.