Fig. 2.

Expression of MamC-RBP on isolated magnetosomes. (A) Immunoblot analysis of proteins solubilized from isolated magnetosomes of WT cells and cells harboring pAP179 (MamC-RBP). Blots were probed with an anti-MamC antibody. (B) Binding of mCherry to RBP-expressing magnetosomes. An mCherry-containing solution was incubated with isolated magnetosomes of WT cells and cells harboring pAP179 (MamC-RBP). Protein fractions from the input (In), supernatant (S), and bound magnetosome particles (M) were resolved by SDS-PAGE, blotted to a nitrocellulose membrane, Ponceau stained (top), and probed with an anti-mCherry antibody (bottom). M, molecular mass.