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Semicontinuous culture of MSR-1 cells in a 7.5-liter fermentor. (A) The maximal OD565 was 25.5 at the end of stage 1 and 13.8 at the end of stage 2. (B) Carbon (▪) and nitrogen (▵) sources were supplied at concentrations suitable for cell growth. (C) The Fe2+/Fe3+ concentration increased during both stages. (D) The Cmag (▵) increased initially and decreased later during each stage. The dO2 (▪) was controlled at 0% starting at 12 h.
