Table 1.

Alkene formation during batch growth of A. tertiaricarbonis L108, M. petroleiphilum PM1, and Methylibium sp. strain R8 on various substrates

Growth substrate	Strain	Amt of substrate turnover (μmol)	% Isobutene formationa	% Isoamylene formationb
MTBE	L108	74 ± 12	0.33 ± 0.07
	R8	71 ± 3	0.25 ± 0.01
	PM1	56 ± 3	0.11 ± 0.01
	PM1	114 ± 17	0.12 ± 0.04
ETBE	L108c	25 ± 3	0.17 ± 0.09
TBA	L108	71 ± 7	0.29 ± 0.03
	R8	89 ± 13	0.26 ± 0.03
	PM1	118 ± 3	0.16 ± 0.02
MPD, 2-HIBA, lactic acid, or fructose	L108d	30-120	≤0.03
TAME	L108	61 ± 18		38 ± 4
	R8	68 ± 5		21 ± 1
	PM1	74 ± 4		4.5 ± 0.7
	PM1	26 ± 6		4.3 ± 0.2
TAA	L108	104 ± 22		9 ± 1
	R8	62 ± 15		22 ± 4
	R8	9 ± 2		27 ± 3
	PM1	130 ± 10		2.5 ± 0.8
3,3-HMB, 2,2-HMB, lactic acid, or fructose	L108d	20-140		≤0.03

^aPercentage of substrate turnover.

^bPercentage of substrate turnover of beta and gamma isomers.

^cNot applicable for strains PM1 and R8.

^dNot tested with strains PM1 and R8.