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Quantitative PCR analysis of bacterial functional groups of the nitrogen cycle. AOB are represented by the ammonium monooxygenase gene (amoA), DNB are represented by the nitrite reductase gene (nirS [nirK was present at <1% throughout]) and the nitrous oxide reductase gene (nosZ), and AMX were detected using a group-specific primer set for the AMX 16S rRNA gene. All data were normalized against qPCR using general bacterial 16S rRNA gene primers.
