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. 2000 Apr 15;14(8):994–1004.

Figure 1.

Figure 1

Structure of GFAP–Cre transgene and characterization of the transgenic mice. (A) Partial structure of the GFAP–Cre transgene. (B) Northern blot analysis of Cre expression on poly(A)+ RNA from three independent transgenic lines. A 1.6-kb mRNA corresponding to the predicted transcript from the GFAP–Cre transgene was detected in Tg2 and Tg6 mice. Control hybridization with hGAPDH probe shows an equal loading of RNA in each lane. (C) Genomic structure of the transgene in the ACZL mice. Cre-mediated recombination leads to juxtaposition of the lacZ gene to the chicken β-actin promoter. (D) PCR analysis of recombination on GFAP–Cre;ACZL double transgenic mice. A 580-bp product for the recombined allele is present in brain, muscle (likely originating from peripheral myelinated nerve fibers), and intestine (enteric glia). The upper band represents the nonrecombined allele. The location of the primers AG2 and Z3 is illustrated in C.