Copy numbers of hcnC gene transcripts expressed by each RNA isolation protocol (A) and for each soil clay content (B) for 0 and 7 days postinoculation with Pseudomonas sp. LBUM300. Black bars, light-gray bars, and dark-gray bars represent soil of low, medium, and high clay content, respectively. Letters indicate significant differences at P < 0.05. All values are means ± standard errors of results from 4 and 24 isolations (A and B, respectively), expressed in hcnC copy numbers per gram of soil.