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Immunoprecipitation of BRCA1-associated proteins and identification by mass spectrometry. (A) Immunoprecipitates of BRCA1-associated proteins using antibodies Ab80 and Ab81 were resolved on a 4%–20% gradient SDS–polyacrylamide gel and stained with Coomassie blue. Labeled protein bands were identified by mass spectrometry. (B) A representative MS/MS spectrum that identifies the 160-kD band from Ab80 immunoprecipitate as the RecQ DNA helicase BLM.
