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. 2000 Apr 15;14(8):927–939.

Figure 6.

Figure 6

Colocalization of RAD50, MRE11, BRCA1, and BLM following treatment of cells with HU. Asynchronous MCF7 cells were treated with 1 mm HU for 4–8 hr. Cells were fixed and stained with the following antibodies: (A–C) anti-RAD50 (13B3, GeneTex) and anti-BRCA1 (Ab-2, Neomarkers); (D–F) anti-MRE11 (12D, GeneTex) and anti-BRCA1 (Ab-2 NeoMarkers); (G–I) anti-MRE11 (12D7 GeneTex); and affinity-purified polyclonal anti-BLM; (J–L) anti-RAD50 (13B3, GeneTex) and affinity-purified polyclonal anti-BLM. Confocal images were captured at 1260× magnification. It should be noted that the majority of cells after HU treatment did not appear significantly different from the untreated cells. However, ∼10% showed redistribution into nuclear foci as represented in these images.