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. 2011 Aug;10(8):1034–1042. doi: 10.1128/EC.05060-11

Fig. 3.

Fig. 3.

Farnesol and 3-oxo-C12-homoserine lactone directly inhibit Cyr1p but not other soluble adenylyl cyclases. The activity of purified recombinant C. albicans Cyr1p (fragment encoding amino acids 1166 to 1571) was assayed with 5 mM ATP and 5 mM MgCl2 in the presence of the indicated concentrations of farnesol [▪, (E,E)-farnesol; ▴, isomeric mixture of farnesol] (A), C12-HSL (B), dodecanol (C), and of various bicarbonate concentrations [▪, solvent control; ▴,16.65 μM E,E-farnesol; ⧫, 50 μM E,E-farnesol] (D). (E) Activity of purified glutathione S-transferase (GST)-truncated rat sAC, in the presence of E,E-farnesol. (F) Activity of purified CyaC in the presence of E,E-farnesol. Data are expressed as percentage of activity in the presence of vehicle alone (ethanol for farnesol and dodecanol and DMSO for C12-HSL), and values are averages derived from four independent experiments, each performed in duplicate. Error bars represent standard error of the mean.