Fig. 4.

RBP74 transcript levels and endocytic location. (A) Abundance of RBP74 mRNA in bloodstream (filled bars) and insect (open bars) forms was assayed by quantitative RT-PCR using tubulin as an internal standard. RBP74 is significantly upregulated in the bloodstream form (*, P < 0.05). Error bars are standard errors from experiments performed in triplicate. (B) Location of HA epitope-tagged RBP74 in bloodstream cells compared to the location of early and recycling endosomes by immunostaining of Rab5A or Rab11. Left panels, RBP74 stained with mouse anti-HA antibody (red); center panels, rabbit polyclonal anti-Rab5 (upper panel) or anti-Rab11 (lower panel) (green). DNA was visualized with DAPI (blue, right panels, merge). Bar, 2 μm.