Fig. 3.

(A) Map of the insert from plasmid pZCF36K2, which contains the wild-type MRR1 gene and served as the basis for the generation of MRR1 genes with C-terminal truncations and internal deletions. (B) MDR1 promoter activity in transformants of the reporter strain CAG48MRR1M4B expressing a wild-type copy of MRR1 or C-terminally truncated derivatives (the first and last amino acids in the encoded proteins are indicated). Strains were grown in the absence or presence of benomyl, and the mean fluorescence of the cells was determined by flow cytometry. The results obtained with two independent transformants are shown in each case (means and standard deviations from three experiments). The parental strain CAG48MRR1M4B (mrr1Δ) was included as a control.