Table 1.
Plasmids used in this study
| Name | Description of encoded protein |
|---|---|
| Control plasmids | |
| pZCF36K2 | Wild-type Mrr1 (amino acids 1-1108) |
| pZCF36K3 | Hyperactive Mrr1 with P683S gain-of-function mutation |
| pZCF36E2 | Wild-type Mrr1 expressed from the ADH1 promoter |
| pZCF36EH2 | HA-tagged Mrr1 expressed from the ADH1 promoter |
| pTET52 | TetR expressed from the ADH1 promoter |
| pTET53 | TetR-Gal4AD fusion expressed from the ADH1 promoter |
| Plasmids encoding Mrr1DB-Gal4AD fusion proteins (expressed from the ADH1 promoter) | |
| pZCF36DBH2 | HA-tagged Gal4AD fused to Mrr1 amino acids 1-128 |
| pZCF36DBH4 | HA-tagged Gal4AD fused to Mrr1 amino acids 1-121 |
| pZCF36DBH5 | HA-tagged Gal4AD fused to Mrr1 amino acids 1-106 |
| pZCF36DBH6 | HA-tagged Gal4AD fused to Mrr1 amino acids 1-90 |
| Plasmids encoding TetR-Mrr1 fusion proteins (expressed from the ADH1 promoter) and containing a Ptet-caGFP reporter fusion | |
| pZCF3667-1108-TETR | Mrr1 amino acids 67-1108 fused to TetR |
| pZCF3691-1108-TETR | Mrr1 amino acids 91-1108 fused to TetR |
| pZCF36104-1108-TETR | Mrr1 amino acids 104-1108 fused to TetR |
| pZCF36122-1108-TETR | Mrr1 amino acids 122-1108 fused to TetR |
| pZCF36144-1108-TETR | Mrr1 amino acids 144-1108 fused to TetR |
| pZCF36168-1108-TETR | Mrr1 amino acids 168-1108 fused to TetR |
| pZCF36269-1108-TETR | Mrr1 amino acids 269-1108 fused to TetR |
| pZCF36524-1108-TETR | Mrr1 amino acids 524-1108 fused to TetR |
| pZCF361001-1108-TETR | Mrr1 amino acids 1001-1108 fused to TetR |
| pZCF361051-1108-TETR | Mrr1 amino acids 1051-1108 fused to TetR |
| pZCF3667-526-TETR | Mrr1 amino acids 67-526 fused to TetR |
| pZCF36269-812-TETR | Mrr1 amino acids 269-812 fused to TetR |
| Plasmids encoding C-terminally truncated Mrr1 | |
| pZCF36K2ΔC1066 | Mrr1 amino acids 1-1066 |
| pZCF36K2ΔC1040 | Mrr1 amino acids 1-1040 |
| pZCF36K2ΔC1031 | Mrr1 amino acids 1-1031 |
| pZCF36K2ΔC1015 | Mrr1 amino acids 1-1015 |
| pZCF36K2ΔC1001 | Mrr1 amino acids 1-1001 |
| pZCF36K2ΔC955 | Mrr1 amino acids 1-955 |
| pZCF36K2ΔC952 | Mrr1 amino acids 1-952 |
| pZCF36K2ΔC948 | Mrr1 amino acids 1-948 |
| pZCF36K2ΔC947 | Mrr1 amino acids 1-947 |
| pZCF36K2ΔC946 | Mrr1 amino acids 1-946 |
| pZCF36K2ΔC945 | Mrr1 amino acids 1-945 |
| pZCF36K2ΔC944 | Mrr1 amino acids 1-944 |
| pZCF36K2ΔC933 | Mrr1 amino acids 1-933 |
| pZCF36K2ΔC911 | Mrr1 amino acids 1-911 |
| pZCF36K2ΔC812 | Mrr1 amino acids 1-812 |
| pZCF36K2ΔC706 | Mrr1 amino acids 1-706 |
| pZCF36K2ΔC366 | Mrr1 amino acids 1-366 |
| Plasmids encoding Mrr1 with internal deletions | |
| pZCF36K2Δ342-381 | Mrr1 lacking amino acids 342-381 |
| pZCF36K2Δ873-896 | Mrr1 lacking amino acids 873-896 |
| pZCF36K2Δ101-150 | Mrr1 lacking amino acids 101-150 |
| pZCF36K2Δ151-200 | Mrr1 lacking amino acids 151-200 |
| pZCF36K2Δ201-250 | Mrr1 lacking amino acids 201-250 |
| pZCF36K2Δ251-300 | Mrr1 lacking amino acids 251-300 |
| pZCF36K2Δ301-350 | Mrr1 lacking amino acids 301-350 |
| pZCF36K2Δ351-400 | Mrr1 lacking amino acids 351-400 |
| pZCF36K2Δ401-450 | Mrr1 lacking amino acids 401-450 |
| pZCF36K2Δ451-500 | Mrr1 lacking amino acids 451-500 |
| pZCF36K2Δ501-550 | Mrr1 lacking amino acids 501-550 |
| pZCF36K2Δ551-600 | Mrr1 lacking amino acids 551-600 |
| pZCF36K2Δ601-650 | Mrr1 lacking amino acids 601-650 |
| pZCF36K2Δ651-700 | Mrr1 lacking amino acids 651-700 |
| pZCF36K2Δ701-750 | Mrr1 lacking amino acids 701-750 |
| pZCF36K2Δ751-800 | Mrr1 lacking amino acids 751-800 |
| pZCF36K2Δ801-850 | Mrr1 lacking amino acids 801-850 |
| pZCF36K2Δ851-900 | Mrr1 lacking amino acids 851-900 |
| pZCF36K2Δ901-950 | Mrr1 lacking amino acids 901-950 |
| pZCF36K2Δ951-1000 | Mrr1 lacking amino acids 951-1000 |
| pZCF36K2Δ1001-1050 | Mrr1 lacking amino acids 1001-1050 |
| pZCF36K2Δ951-1050 | Mrr1 lacking amino acids 951-1050 |
| pZCF36K2Δ129-1000 | Mrr1 lacking amino acids 129-1000 |
| pZCF36K2Δ129-1050 | Mrr1 lacking amino acids 129-1050 |
| pZCF36DB-751-952 | Mrr1 amino acids 751-952 fused to amino acids 1-128 |
| pZCF36DB-851-952 | Mrr1 amino acids 851-952 fused to amino acids 1-128 |