Table 3.

Three representative examples of relative quantification of wall proteins of planktonic C. albicans cultures with and without fluconazole

Protein name and position (aa)a	Protein function (length [aa])	Tryptic peptide	FCZ 14N/15N ratio by replicateb				Control 14N/15N ratio by replicateb
			R1	R2	R3	R4	R1	R2	R3	R4
Cht2	Chitinase, GH18 domain (583)
99–121		TVLLSLGGGVGDYGFSDVASATK	0.22	0.19	0.22	0.22	1.08	1.19	1.03	0.82
122–129		FADTLWNK	0.18	0.21	0.24	0.22	1.26	1.25	1.09	0.84
178–200		NYFLSAAPQBPYPDASLGDLLSK	0.26	0.22	0.27	0.25	1.26	1.15	ND	0.91
240–258		LFVGVPATSNIAGYVDTSK	0.24	0.20	0.24	0.21	1.07	1.16	1.02	0.78
259–267		LSSAIEEIK	0.26	0.20	0.23	0.24	1.06	1.13	0.81	0.88
268–290		BDSHFAGVSLWDASGAWLNTDEK	ND	ND	ND	ND	1.20	low	ND	0.78
291–299		GENFVVQVK	0.16	0.20	0.24	0.23	1.04	1.17	0.98	0.83
Avg per replicate			0.21	0.20	0.24	0.23	1.14	1.17	0.98	0.83
Avg per condition				0.22 ± 0.08				1.03 ± 0.01
FCZ/control ratio						0.22 ± 0.08
Crh11	Transglycosylase (453)
22–28		DTBNPLK	1.38	8.18	6.97	ND	ND	ND	ND	ND
29–45		SSDBSPVPALGSSFLEK	3.87	ND	4.79	6.20	0.79	1.45	1.40	1.27
46–58		FDNGLGPHFESLK	2.93	2.74	3.52	5.01	0.59	1.11	1.26	1.08
46–59		FDNGLGPHFESLKK	3.23	3.11	5.42	9.08	low	1.05	ND	ND
60–75		QGTIDSGSNGLSLTMK	4.74	ND	ND	low	ND	ND	ND	ND
77–84		RFDNPSFK	ND	3.60	4.14	5.57	0.78	1.48	1.15	low
78–84		FDNPSFK	3.48	3.86	4.92	5.95	0.74	1.45	ND	1.16
85–93		SNFYIMFGR	3.52	3.52	4.31	5.20	0.83	1.43	1.26	1.13
148–158		GGYHDIANPLK	2.22	2.38	3.62	ND	0.25	0.49	ND	ND
159–169		DYHTYVIDWTK	3.51	3.52	4.50	5.62	0.85	1.39	1.30	1.15
170–182		DAVTWSVDGSVIR	4.82	3.43	4.09	6.02	1.36	1.37	1.36	1.22
236–246		SVLVADYSSGK	3.59	3.64	4.53	5.31	0.84	1.43	1.34	1.17
247–261		QYSYSDQSGSWESIK	ND	low	7.76	8.81	ND	ND	ND	ND
271–279		YDQAQDDIK	6.82	ND	ND	ND	ND	ND	ND	ND
Avg per replicate			3.44	3.59	4.75	6.15	0.72	1.21	1.29	1.17
Avg per condition				4.48 ± 0.13				1.10 ± 0.63
FCZ/control ratio						4.05 ± 0.64
Ecm33	Role in wall integrity (423)
139–168		TGLTAGITSAESVVISDTGLSSLTGINVFK	ND	0.55	0.50	ND	ND	ND	ND	0.60
246–262		VELAELTSIGNSLTINK	0.58	0.56	0.56	0.67	0.43	0.45	0.53	0.51
263–273		NDDLTELDFPK	0.54	0.62	0.57	0.64	0.43	0.49	0.45	0.49
276–290		TIGGALQISDNSELR	0.86	ND	0.49	0.65	0.33	0.32	0.50	0.48
291–297		SFSGFPK	0.67	0.67	0.67	0.61	0.49	0.50	0.48	0.51
322–329		VSGGFILK	0.33	0.59	0.58	0.61	0.43	0.45	0.48	0.46
334–341		LSBSAFNK	0.60	0.61	0.67	0.64	0.52	0.48	0.53	0.51
Avg per replicate			0.57	0.60	0.57	0.64	0.43	0.44	0.49	0.51
Avg per condition				0.60 ± 0.02				0.47 ± 0.01
FCZ/control ratio						1.26 ± 0.03

^aaa, amino acids. For full information about all proteins from planktonic and surface-grown cultures see Table S3 in the supplemental material.

^bR1 to R4, replicates 1 to 4; ND, not detected in the ¹⁴N-labeled cultures; low, peptides of an intensity too low to be suitable for quantification. Average values and the FCZ/control ratio are means ± standard errors.