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. 2011 Aug;10(8):1062–1070. doi: 10.1128/EC.00007-11

Fig. 1.

Fig. 1.

Yeast two-hybrid and co-IP assays for the interaction of PMK1 with PIC1 and PIC5. (A) Yeast transformants expressing the PMK1 bait and PIC1 or PIC5 prey constructs were assayed for growth on SD-Leu-Trp-His (SD-His) plates and β-galactosidase (LacZ) activities. Positive and negative controls are marked with + and −. (B) Western blot analysis with total proteins isolated from M. oryzae transformants expressing the PIC1-3xFLAG and PIC5-3xFLAG constructs and proteins eluted from the anti-FLAG M2 beads. The presence of Pmk1 was detected with an anti-Pmk1 antibody (2). Total proteins isolated from the wild-type M. oryzae strain (70-15) and detection with an anti-actin antibody were included as controls.