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Penetration assays with rice leaf sheaths. Extensive invasive hyphae were developed by the wild type (70-15) and the pic5 complemented transformant (MF2) by 48 h after inoculation. Most of the appressoria formed by the pic5 mutant strains ML4 and ML13 failed to penetrate or had only limited growth of primary invasive hyphae. Bar = 10 μm.
