Fig. 5.

Localization of SRA to the flagellar pocket is not critical to resistance. (A and B) Uptake of Alexa Fluor 488-TLF (green) at 37°C for 1 min postbinding in SRA-Ty (A) and SRAΔGPI (B) cells. SRA was visualized via anti-Ty staining (red). White arrowheads indicate colocalization. (C) Untransfected T. brucei brucei 221, T. brucei brucei 221 SRA-Ty, and T. brucei brucei 221 SRAΔGPI cells were incubated with increasing concentrations of purified TLF-1. Percent lysis was determined after 2 h at 37°C.