Table 2.
Known and putative signal transduction pathway genes required for cell fusion
| Gene name (locus no.)a | Mutant characteristic |
Verification by complementation exptb | Predicted function of proteinc | ||
|---|---|---|---|---|---|
| CAT formation | Growth (mm/h) | Phenotyped | |||
| mik-1 (NCU02234) | No | 0.33 | Fc Pd sickly microconidial | Yes† | MAPKK kinase |
| mek-1 (NCU06419) | No | 0.08 | Fc Pd sickly | Yes† | MAPK kinase |
| mak-1 (NCU11376) | No | 0.25 | Fc Pd sickly microconidial | Yes† | MAP kinase |
| nrc-1 (NCU06182) | No | 0.21 | Fc Pd sickly | Yes† | MAPKK kinase |
| mek-2 (NCU04612) | No | 1.14 | Fc Pd sickly | Yes† | MAPK kinase |
| mak-2 (NCU02393) | No | 0.21 | Fc Pd sickly | Yes† | MAP kinase |
| rac-1 (NCU02160)* | No | 0.01 | Colonial growth Pd | No | MAPK pathway |
| pp2A (NCU06563)* | No | 0.58 | Fc Pd | No | MAPK pathway |
| so/ham-1 (NCU02784) | No | 0.83 | Fc Pd | Yes† | Signaling activity |
| ham-2 (NCU03727) | No | 0.38 | Fc Pd | Yes† | Signaling pathway |
| ham-3 (NCU08741) | No | 0.17 | Fc late protoperithecia | No† | Signaling pathway |
| ham-5 (NCU01789) | No | 0.83 | Fc Pd | No† | MAPK pathway |
| mob3 (NCU07674) | No | 0.42 | Fc Pd | Yes†** | Signaling pathway |
| ham-9 (NCU07389)* | No | 1.82 | Fc Pd | Yes** | Signaling pathway |
a
Asterisks mark genes newly identified by the screening procedure as required for cell fusion.
b
Daggers mark genes previously identified as required for cell fusion. Double asterisks mark complementation experiments carried out in conjunction with this study.
c
MAPK, MAP kinase; MAPKK, MAPK kinase.
d
Fc, flat conidiation phenotype; Pd, delayed or absent protoperithecium development.