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. 2011 Aug;10(8):1043–1052. doi: 10.1128/EC.05071-11

Fig. 6.

Fig. 6.

Chemical complementation by potassium acetate in G. zeae strains. (A) Self-fertility of the G. zeae strains on carrot agar 10 days after sexual induction. Each strain was inoculated on carrot agar (upper), carrot agar supplemented with 40 mM potassium acetate (middle), and carrot agar supplemented with both 40 mM potassium acetate and 30 μM zearalenone (bottom). The white arrows indicate immature perithecia. Scale bar, 0.5 mm. (B) Mycelial growth of G. zeae strains in liquid minimal medium supplemented with 2% of glucose (MMG) 5 days after inoculation. The conidium suspension of each strain was inoculated in MMG (upper), MMG supplemented with 40 mM potassium acetate (middle), and MMG supplemented with both 40 mM potassium acetate and 30 μM ZEA (bottom). WT, G. zeae wild-type strain GZ3639; Δacs1, ACS1 deletion mutant; Δacs2, ACS2 deletion mutant; Δacl2, ACL2 deletion mutant; Δacl2 Δacs1, double deletion mutant of ACL2 and ACS2; Δacl2 ACS2p, ACS2 promoter-replaced mutant in the Δacl2 strain. Ac, exogenous treatment of 40 mM potassium acetate; MMGAc, MMG supplemented with 40 mM potassium acetate; ZEA, exogenous treatment of 30 μM ZEA.