Fig. 3.
Pgd− and Pgd− Oat− L. monocytogenes strains induce increased cytosolic and vacuolar cytokine responses dependent on the presence of host lysozyme. A total of 106 wt (black bars) or LysM− (gray bars) BMM were infected with wt or lysozyme-sensitive L. monocytogenes at an MOI of 1 for 4 h. LLO− infections were also performed at an MOI of 1. RNA was harvested, and IL-1β (A), IL-12 (B), and IFN-β (C) transcripts were measured relative to those of β-actin by quantitative PCR. Error bars represent standard deviations of the means determined in triplicate. Data are representative of at least 3 independent experiments with similar results. One-way analysis of variance (ANOVA) and Tukey's post hoc test were used to analyze the significance of infections in either wt or LysM− BMM (dashed lines). Student's t test was used to analyze significance for each bacterial strain. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant.